diff --git a/notebooks/Jungfrau/Jungfrau_Gain_Correct_and_Verify_NBC.ipynb b/notebooks/Jungfrau/Jungfrau_Gain_Correct_and_Verify_NBC.ipynb
index f915003c5f1069a34a4924c16cb4b880749b67d7..b679a9aa209311430cf1e095292b6005b3b43c67 100644
--- a/notebooks/Jungfrau/Jungfrau_Gain_Correct_and_Verify_NBC.ipynb
+++ b/notebooks/Jungfrau/Jungfrau_Gain_Correct_and_Verify_NBC.ipynb
@@ -60,7 +60,7 @@
     "\n",
     "# Parameters for ROI selection and reduction\n",
     "roi_definitions = [-1]  # List with groups of 6 values defining ROIs, e.g. [3, 120, 180, 200, 550, -2] for module 3 (JNGFR03), slice 120:180, 200:550, average along axis -2 (slow scan, or -1 for fast scan)\n",
-    "roi_threshold = -1  # Corrected pixels below the threshold will be excluded from ROI projections. Set to -1 to include all pixels.\n",
+    "roi_threshold = 2  # Corrected pixels below the threshold will be excluded from ROI projections. Set to -1 to include all pixels.\n",
     "\n",
     "def balance_sequences(in_folder, run, sequences, sequences_per_node, karabo_da):\n",
     "    from xfel_calibrate.calibrate import balance_sequences as bs\n",